Comedians Desiree Burch and Guz Khan join Alex Brooker and Josh Widdicombe at the famous animal attraction. During a magical and high-spirited night, they hang out with the giraffes, feed the camels and endure the company of spiders, as they freewheel around the zoo in a golf cart and stay overnight in the zoo's lodges. As the night progresses, the friends get a glimpse into what the animals get up to after dark, and even find themselves inadvertently spying on some amorous lions, as they make the most of this once-in-a-lifetime chance to enjoy the zoo without any other members of the public present.